TGAS-MAN Research

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Site and Infrastructure
Phase 1: 2005-2017
Phase 2: 2018-2021
Support and Funding Sources

TGAS-MAN Origins

When Dr. Mario Tenuta began his career at the University of Manitoba, he recognized that there was a lack of information on whole year budgets of nitrous oxide (N2O) and carbon dioxide (CO2) emissions, especially together, from cropping systems in the prairies and elsewhere in Canada. This knowledge gap was of great significance as N2O and CO2 are the major greenhouse gasses emitted from agricultural soil.

Unfortunately, emissions are very episodic and therefore often missed or not fully captured using traditional sampling methods which are labor-intensive and conducted intermittently. Traditional methods, such as using static-vented chambers, are also challenging to deploy and execute during spring thaw, which is a particularly important time in Prairie Canada for N2O emissions.

Traditional gas emissions sampling methods are not feasible in conditions like these, but the micrometeorological stations and trace gas analyzer at TGAS-MAN are able to provide continuous nitrous oxide and carbon dioxide flux-gradient data throughout the entire spring thaw ©Krista Hanis-Gervais

To further complicate the knowledge gap, about ten years of data collection is required to thoroughly quantify changes in soil organic matter levels resulting from farming practices. Short-term studies, while easier to conduct, are insufficient to determine the consequences of farming practices on carbon sequestration.

As a result of the knowledge gap and the underlying difficulties with addressing it, Dr. Tenuta realized that a micrometeorological system to monitor whole-field fluxes of N2O and CO2 was needed for Prairie Canada. In 2005, as part of the new National Centre for Livestock and the Environment (NCLE) initiative and with funding from the Canada Foundation for Innovation (CFI), under the leadership of Dr. Mario Tenuta and with technical assistance from Dr. Brian Amiro (University of Manitoba) and help from Applied Soil Ecology Lab technicians, Trace Gas Manitoba (TGAS-MAN) was established.

Dr. Brian Amiro and several technicians lay out gas lines that will connect each TGAS-MAN micrometeorological station with the central trace gas analyzer ©Mario Tenuta.

TGAS-MAN was envisioned to be a long-term site to test farming practices and their impact on greenhouse gas emissions, with the goal of providing empirical data to guide best management practices to lower emissions. Which farming practices are tested have changed over time to address current questions as technology and farming practices evolved.

Site & Infrastructure

TGAS-MAN is located in the Red River Valley approximately 16 km south of Winnipeg, MB, Canada. The area is a glaciolacustrine clay floodplain with near-level topography, extreme humid-continental climate, and soils predominantly of the Red River (Gleyed Humic Certisol) and Osborne (Gleysolic Humic Vertisol) soil series. The field is imperfectly drained and periodic flooding and excessive soil moisture, often around snow melt in spring or heavy early growing season rains, are not uncommon.

The future location of Trace Gas Manitoba, one year before the field site was established. This satellite image, taken in early fall, makes it easy to see the imperfect drainage and prairie pothole wetlands that lasted through summer.

The TGAS-MAN field site is located in a 30-ha farmed field and consists of four 200m x 200m (4-ha) experimental plots arranged in a 2×2 grid.

Each plot is outfitted with its own micrometeorology station, which monitors environmental conditions such as wind speed and direction, air temperature, and soil moisture and temperature.

Each TGAS-MAN micrometeorology station includes a 3-D sonic anemometer and gas intakes ©Krista Hanis-Gervais

At the epicenter of TGAS-MAN is the Tunable Diode Laser absorption spectrophotometer (Campbell TGAS100A) trace gas analyzer. Housed in an insulated trailer located at the junction of the four experimental plots, the trace gas analyzer continuously measures direct emissions from the TGAS-MAN plots for flux-gradient estimation of N2O and CO2 from soil.

Gas intakes at two heights spaced 0.5m apart on each micrometeorological station alternately provide air samples to the analyzer, switching every 15 seconds. Only one plot is sampled at a time, for 30 minutes, yielding a maximum of 12 half-hourly concentration differences per day per plot.

A tunable diode laser absorption spectrophotometer (Campbell TGAS100A) trace gas analyzer continuously measures direct emissions of N2O and COfrom soil in the TGAS-MAN plots
©Krista Hanis-Gervais

A fully-equipped weather station is located 25m southwest of the trailer, which measures wind speed and direction, air temperature and relative humidity, precipitation, barometric pressure, snow depth, soil temperature and moisture content at various depths, incoming solar radiation, and photosynthetically active photon flux density.

The central TGAS-MAN weather station ©Krista Hanis-Gervais
TGAS-MAN facilities, including the central weather station, are active in winter ©Marliese Peterson

PHASE 1: 2005-2018

Prior to establishment of TGAS-MAN, the field site was under a C3 crop rotation (primarily cereals and oilseeds) and managed using conventional/intensive tillage techniques. Set-up and monitoring of the TGAS-MAN experimental plots began in the fall of 2005 following a Fallow Year during which no crop was planted or fertilizer used and weed management was by tillage and chemical means.

Over the next thirteen years, micrometeorological conditions and emissions were monitored multiple times daily, almost without interruption. Field conditions during the growing season were also monitored: soil samples were collected from all plots several times throughout each growing season and analyzed for gravimetric moisture content and nitrate (NO3) and ammonium (NH4) content; biomass samples were collected annually at harvest and analyzed for yield and total carbon and nitrogen content. Crop rotations during Phase 1 of TGAS-MAN included alfalfa + grass, barley, canola, corn, faba bean, soybean, and spring wheat.

YearPlots 1 and 4Plots 2 and 3
Fallow YearFallow Year
2007Faba BeanFaba Bean
2008Alfalfa + GrassSpring Wheat
2009Alfalfa + GrassCanola
2010Alfalfa + GrassBarley
2011Alfalfa + GrassSpring Wheat
2019OatsOats w Fall Rye
Cover Crop

During Phase 1, several short-term projects were conducted. These involved experimental manipulation of farming practices on the TGAS-MAN plots, such as using different tillage intensities, annual vs. perennial cropping systems, and timing of fertilizer application. Data collected during these experiments was also used to address other research objectives:

  • Tenuta, M., Amiro, B., Gao, X., Wagner-Riddle, C., and Gervais, M.(2019) Agricultural management practices and environmental drivers of nitrous oxide emissions over a decade for an annual and an annual-perennial crop rotation. Agricultural and Forest Meteorology (accepted).
  • Amiro, B., Tenuta, M., Gervais, M.Glenn, A., and Gao, X. (2017). A decade of carbon flux measurements with annual and perennial crop rotations on the Canadian Prairies. Agricultural and Forest Meteorology. 247: 491-502. (PREVIEW)
  • Uzoma, K.C., Smith, W.N., Grant, B.B., Desjardins, R.L., Gao, X.Hanis, K., Tenuta, M., Goglio, P., and Li, C. (2015). Assessing the effects of agricultural management on nitrous oxide emissions using flux measurements and the CAN-DNDC model. Agriculture, Ecosystems and Environment. 206: 71-83. (PREVIEW)
  • Gilmanov, T.G., Baker, J.M., Bernacchi, C.J., Billesbach, D.P., Burba, G.G., Castro, S., Eugster, W., Fischer, M.L., Gamon, J.A., Gebremedhin, M.T., Glenn, A.J., Griffis, T.J., Hatfield, J.L., Heuer, W.L., Howard, D.M., Leclerc, M.Y., Loescher, H.W., Matamala, R., Meyers, T.P., Phillips, R.L., Prueger, J.H., Suyker, A.E., Tenuta, M., and Wylie, B.K. (2014). Productivity and carbon dioxide exchange of leguminous crops: Estimates from flux tower measurements. Agronomy Journal. 106: 545-559. (PREVIEW)
  • Gilmanov, T.G., Wylie, B.K., Tieszen, L.L., Tilden, P.M., Baron, V.S., Bernacchi, C.J., Billesbach, D.P., Burba, G.G., Fischer, M.L., Glenn A.J., Hanan, N.P., Hatfield, J.L., Heuer, M.W., Hollinger, S.E., Howard, D.M., Matamala, R., Prueger, J.H., Tenuta, M., and Young, D.G. (2013). CO2 uptake and ecophysiological parameters of the grain crops of mid-continent North America: Estimates from flux tower measurements. Agriculture, Ecosystems and Environment. 164: 162-175. (PREVIEW)
Conventional vs. Reduced Tillage (2006-2008)

A comparison of the effects of conventional/intensive tillage vs reduced tillage on CO2 exchange was conducted during the 2006 through 2008 growing seasons. All plots were planted with corn and faba bean in 2006 and 2007, respectively. In 2008, plots 2 and 3 were planted with spring wheat while plots 1 and 4 were planted with a perennial forage crop and thus omitted from the study. To compare the two farming practices, plots 1 and 4 were managed using reduced tillage while plots 2 and 3 were managed with conventional/intensive tillage. The flux-gradient method was used to determine the net exchange of CO2 between the soil-crop system and the lower atmosphere.

  • Glenn, A.J., Amiro, B.D., Tenuta, M., Stewart, S.E., and Wagner-Riddle, C. (2010). Carbon dioxide exchange in a northern prairie cropping system over three years. Agricultural and Forest Meteorology. 150: 908-918. (PREVIEW)
Crop Residue Carbon and Soil Respiration (2006-2007)

To determine the relative contributions of crop residue carbon and soil organic carbon pools to respiration in a northern agroecosystem where the non-growing season is long, carbon dioxide flux-gradient data from all plots were examined during two non-growing periods: post-harvest to pre-freeze in the fall of 2006 and post snowmelt and pre-field operations in the spring of 2007.

  • Glenn, A.J., Amiro, B.D., Tenuta, M., Wagner-Riddle, C., Drewitt, G., and Warland, J. (2011). Contribution of crop residue carbon to soil respiration at a northern Prairie site using stable isotope flux measurements. Agricultural and Forest Meteorology. 151: 1045-1054. (DOWNLOAD)
Nitrous Oxide Emissions from an Annual Crop Rotation (2006-2008)

To address the lack of multi-year studies of N2O emissions from poorly drained floodplain soil, emissions were monitored at TGAS-MAN from 2006 through to 2008, during which time all plots were planted with a corn, faba bean, and spring wheat rotation. Because of the concurrent tillage study, this project was also able to investigate the effects of different tillage practices on N2O emissions.

  • Glenn, A.J., Tenuta, M., Amiro, B.D., Maas, S.E., and Wagner-Riddle, C. (2012). Nitrous oxide emissions from an annual crop rotation on poorly drained soil on the Canadian Prairies. Agricultural and Forest Meteorology. 166-167: 41-49. (PREVIEW)
  • Aaron Glenn – PhD Thesis (2006-2010) Greenhouse Gas Fluxes and Budget for an Annual Cropping System in the Red River Valley, Manitoba, Canada (DOWNLOAD)
Perennial vs. Annual Cropping (2008-2011)

The short-term benefit of including perennial forage in an annual crop rotation on CO2 and N2O emissions was investigated from 2008 through to 2011. During this time, plots 1 and 4 were converted from annual crops to perennial forage composed of alfalfa and timothy grass while plots 2 and 3 continued with the annual crops wheat, canola, and barley. CO2 and N2O fluxes were measured continuously using the flux-gradient micrometeorological method.

  • Maas, S.E.Glenn, A.J., Tenuta, M., and Amiro, B.D. (2013). Net CO2 and N2O exchange during perennial forage establishment in an annual crop rotation in the Red River Valley, Manitoba. Canadian Journal of Soil Science. 93: 639-652. (PREVIEW)
  • Siobhan Maas – MSc Thesis (2009-2011) Perennial Legume Phase and Annual Crop Rotation Influences on CO2 and N2O Fluxes Over Two Years in the Red River Valley, Manitoba, Canada (DOWNLOAD)
Fall vs. Spring-Applied Anhydrous Ammonia (2011-2012)

In 2011 and 2012, Plots 2 and 3 were used to compare the effects of late fall and pre-plant application of anhydrous ammonia on N2O emissions. Spring wheat and corn were planted in 2011 and 2012, respectively, and field-scale flux of N2O was measured using each plot’s micrometeorological station.

  • Tenuta, M., Gao, X., Flaten, D.N., and Amiro, B.D. (2016). Lower nitrous oxide emissions from anhydrous ammonia application prior to soil freezing in late fall than spring pre-plant application. Journal of Environmental Quality. 45(4): 1135-1143. (DOWNLOAD)
TGAS-MAN on May 16, 2011: perennial plots (left) continued with an alfalfa / grass mix while annual plots (right) wait to be seeded with spring wheat. To compare the effects of fall vs. spring-applied anhydrous ammonia on nitrous oxide emissions, fertilizer was applied to the southeast plot in the fall of 2010, while the northeast plot will be fertilized shortly before seeding.
Freeze-Thaw Cycles and Nitrous Oxide Emissions (2006-2014)

Data collected at TGAS-MAN from 2006 to 2014 and at a similar micrometeorological experimental site in Elora, Ontario from 2000 to 2014, was used to investigate the contribution of seasonal freezing to N2O emissions from croplands.

  • Wagner-Riddle, C., Congreves, K.A., Abalos, D., Berg, A.A., Brown, S.E., Ambadan, J.T., Gao, X., and Tenuta, M. (2017). Globally important nitrous oxide emissions from croplands induced by freeze-thaw cycles. Nature Geoscience. 10: 279–283. (PREVIEW)
Continued monitoring under conventional farming practices (2013-2018)

No experimental manipulations were conducted at TGAS-MAN from 2013 through to 2018, though monitoring of emissions and field conditions continued. This period allowed the plots time to recover from manipulations that had taken place during the previous seven growing seasons, in preparation for Phase 2 of TGAS-MAN.

PHASE 2: 2018-2021

On August 29, 2018, a cover crop of Fall Rye was applied without fertilizer and only to the east plots (Plot 2 and 3) of TGAS-MAN, marking the beginning of Phase 2 and a new project investigating the effects of cover cropping on greenhouse gas emissions. On the evening of May 13, 2019, all plots were seeded with oats. The following morning, glyphosate was applied to Plots 2 and 3 to terminate the fall rye. Watch the oats grow with our TGAS-MAN Camera.

Fall Rye, seeded in TGAS-MAN plots 2 and 3 in 2018 survived the long, cold winter; in spring of 2019 all plots were seeded with oats to study the impact of cover crops on soil nitrous oxide and carbon dioxide emissions ©Krista Hanis-Gervais
Cover Cropping Benefit to N2O Emission Reductions and Soil Carbon Storage for Oat Production in the Red River Valley of Manitoba

Funding Source: General Mills

Summary: This project aims to resolve significant gaps in understanding N2O emissions from the increasingly popular practice of cover cropping. Past studies focused on warmer climates where thaw emissions are less important. On the Prairies and in Eastern Canada, thaw can contribute to 20-50% of emissions for conventional systems and, as we recently discovered, > 80% of emissions for organic systems.

The studies here will discover if covers cause reduced thaw emissions by lowering soil NO3 and will determine how low levels need to be to limit emissions. Alternatively, covers may increase C availability to denitrifiers increasing emissions at thaw, particularly with termination before planting. We will learn how soil moisture determines relative N2O and N2 emissions to increased C availability and lower NO3 with covers. Knowing how covers affect soil emissions allows for better forecasting of the national GHG inventory.

The research will also help in reducing the environmental footprint (N2O and CO2 emissions) of oat production in the Red River Valley, an area that General Mills sources most of the oats for its products.

TGAS-MAN Support and Funding Sources

Past and present support and funding for TGAS-MAN has come from a variety of sources:

  • AAFC Agriculture Greenhouse Gas Program (AGGP)
  • AAFC Canadian Agricultural Adaptation Program (CAAP)
  • Bell-MTS
  • BIOCAP Canada
  • Canada Research Chair in Applied Soil Ecology
  • Canadian Fertilizer Institute
  • Canadian Foundation for Innovation grant to NCLE
  • General Mills
  • Manitoba Sustainable Agriculture Practices Program (Government of Manitoba)
  • National Centre for Livestock and the Environment (NCLE)
  • NSERC Discovery Grant Program
  • NSERC Strategic Grant
  • Prairie Improvement Network (formerly Manitoba Rural Adaptation Council)

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